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KMID : 1102020210510010012
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Applied Microscopy
2021 Volume.51 No. 1 p.12 ~ p.12
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Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature
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Janssen Barry G. H.
Najiminaini Mohamadreza
Zhang Yan Min
Omidi Parsa
Carson Jeffrey J. L.
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Abstract
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Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.
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KEYWORD
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Multispectral microscopy, Brightfield microscopy, Fluorescence microscopy, Intravital microscopy
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